WeeFoal™
Urine Pregnancy
Test for Mares
Urine collection
The WeeFoal™ test is best performed on
freshly collected urine obtained at any time of the day. We
like to suggest collecting a sample from first or early
morning void. The test may also be performed on collected
urine which has been stored for up to 48 hours at 4 degrees
centigrade. It does not have to be in sterile conditions. A
wee bit of dirt wont hurt. To see an example of collecting
urine for this test, see our
on-line
example.
Test procedure
The WeeFoal™ test can be performed on a
urine sample collected from 120 days after breeding to day
300. It is not recommended to use the test before 120 days
or after day 300 as results maybe inconclusive. Run the test
at room temperature on a white paper or paper towel in a
well-lighted area. The urine sample and test pack should be
at room temperature before performing the test. The urine
sample must be diluted as described below.
Do not run the test with undiluted urine.
When ready to perform the test, open the foil pouch and
place the test cassette, 2 plastic pipettes and the small
tube of urine diluent on a flat, clean surface. Take one of
the pipettes, squeeze the upper bulb and draw enough of the
urine sample into the pipette to fill the stem completely.
(0.15 ml will fill the stem, and any overdrawn amount will
go into the lower bulb reservoir). Discharge the urine
sample from the pipette stem into the small tube of urine
diluent by gently squeezing the upper bulb (any excess fluid
from the draw will remain in the reservoir). Replace the cap
on the small tube of urine diluent and invert the tube twice
to thoroughly mix the urine with the diluent. Take the
second pipette, put the stem in the tube of diluted urine,
and draw enough of the diluted urine sample into the pipette
to fill the stem completely. Discharge the diluted urine
sample drop by drop from the pipette stem into the round
sample well of the cassette by gently squeezing the upper
bulb (any excess fluid from the draw will remain in the
reservoir).

Allow the sample to soak into the absorbent pad in the
well between drops. Once a few drops have been added to the
sample well, the urine will start to flow along the membrane
in the cassette window. Continue adding drops of diluted
urine until the leading edge of the fluid is ¾ of the way
along the membrane, i.e. has reached the ‘C’ mark on the
plastic housing.
Do not flood the sample well by adding too much sample, or
adding it too quickly.
Wait for 5 to 10 minutes. The test result will generally be
obvious within 5 minutes but occasionally it may take up to
10 minutes for the result to clearly form.

Positive Negative
Interpreting the
result